Cre-mediated recombination removed the neo cassette and the microRNA stem loop from Mir320tm1Mtm. qRT-PCR analysis confirmed the loss of the microRNA. Beta-galactosidase expression is observed in brain and spinal cord of E11.5 embryos. (J:181752)
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Cre-mediated recombination removed the neo cassette and the microRNA stem loop from Mir320tm1Mtm. qRT-PCR analysis confirmed the loss of the microRNA. Beta-galactosidase expression is observed in brain and spinal cord of E11.5 embryos. (J:181752)