The microRNA was replaced with a lacZ gene and neo cassette flanked by neo and FRT sites. Flp-mediated recombination removed the neo cassette. Real-time PCR confirmed the absence of transcript expression in mouse embryonic fibroblasts. (J:180692)
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The microRNA was replaced with a lacZ gene and neo cassette flanked by neo and FRT sites. Flp-mediated recombination removed the neo cassette. Real-time PCR confirmed the absence of transcript expression in mouse embryonic fibroblasts. (J:180692)