A loxP site was inserted upstream of exon 4 followed by a frt-flanked neomycin resistance (neo) cassette and a second loxP site inserted downstream of exon 8 via homologous recombination. Cre-mediated recombination removed exons 4-8 and the neo cassette. Flow cytometric analysis confirmed that LAIR1 could not be detected on T, B, and NK cells derived from splenocytes. (J:180203)