An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette and left exons 2 through 4 floxed. (J:178952)
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An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette and left exons 2 through 4 floxed. (J:178952)