One loxP site was inserted upstream of exon 8 and an FRT flanked PGK-neomycin selection cassette together with a second loxP site was inserted downstream of exon 12. Exons 8-12 were subsequently removed by cre excision. (J:173550)
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One loxP site was inserted upstream of exon 8 and an FRT flanked PGK-neomycin selection cassette together with a second loxP site was inserted downstream of exon 12. Exons 8-12 were subsequently removed by cre excision. (J:173550)