An IRES-creERT cassette, together with an FRT flanked PGK-neo cassette, were inserted into the 3' UTR. The neo selection cassette was subsequently excised by crossing to mice expressing flp recombinase. (J:180047, J:182218)
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An IRES-creERT cassette, together with an FRT flanked PGK-neo cassette, were inserted into the 3' UTR. The neo selection cassette was subsequently excised by crossing to mice expressing flp recombinase. (J:180047, J:182218)