RMCE on KH2 cells inserts an shRNA targeting Rsp19 behind a tetracycline responsive element and CMV promoter replacing the Pou5f1 cassette in Col1a1tm2(tetO-Pou5f1)Jae into the 3'UTR. The level of knock down by this allele following doxycycline treatment is lower compared to mice expressing Col1a1tm2(tetO-RNAi:Rps19)Karl . (J:179085)