Cldn11^tm1.1Ral

A construct containing a promoterless lacZ gene and a The floxed PGK-neomycin resistance gene of the original targeted allele was removed by cre excision. A promoterless lacZ gene remains in place of exon 1 and a portion of intron 1. Northern blot analysis of extracts from brains and testes showed a lack of transcript in homozygous mutant mice. Western blot analysis confirmed an absence of encoded protein in brain homogenates. Expression of the inserted lacZ gene was under the control of the endogenous promoter. (J:59025, J:180429)