A loxP site was inserted upstream of exon 1. A floxed neo cassette with a 3' EGFP gene was inserted downstream of exon 1. Cre-mediated recombination removed the neo cassette and left exon 1 floxed followed by the EGFP gene. (J:178164)
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A loxP site was inserted upstream of exon 1. A floxed neo cassette with a 3' EGFP gene was inserted downstream of exon 1. Cre-mediated recombination removed the neo cassette and left exon 1 floxed followed by the EGFP gene. (J:178164)