A loxP site was inserted upstream of the open reading frame and a floxed neo cassette was inserted downstream of the open reading frame via homologous recombination. Cre mediated recombination removed the open reading frame and the neo cassette.
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A loxP site was inserted upstream of the open reading frame and a floxed neo cassette was inserted downstream of the open reading frame via homologous recombination. Cre mediated recombination removed the open reading frame and the neo cassette.