A "gene trapping" cassette containing IRES, betaGeo and PGK1-puro sequence was inserted in place of part of exon 5 and all of exons 6 and 7. Absence of protein in the heart was verified by immunoblot analysis. (J:178182, J:178185)
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A "gene trapping" cassette containing IRES, betaGeo and PGK1-puro sequence was inserted in place of part of exon 5 and all of exons 6 and 7. Absence of protein in the heart was verified by immunoblot analysis. (J:178182, J:178185)