A loxP site was inserted upstream of exon 9 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 10 via homologous recombination. Flp and cre mediated recombination removed the neo cassette and exons 9 and 10. (J:177443)
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A loxP site was inserted upstream of exon 9 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 10 via homologous recombination. Flp and cre mediated recombination removed the neo cassette and exons 9 and 10. (J:177443)