A cre/ERT2-Frt-Kanamycin-Frt sequence was inserted by recombineering methods (in bacterial cells) in the first methionine position of the Aspm gene located in a BAC (BAC RP-24-26-F8). The Kana selection marker was deleted by activation of Flpe in the EL250 E.coli cells. Purified BAC DNA was injected into FVB zygotes. No founder information is available. (J:172348)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
FVB
--
Insertion
--
--
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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