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A targeting construct containing a cre-IRES-mCherry sequence was inserted upstream of the ATG translation initiation site of Il9 via homologous recombination into a BAC clone (RP24-282A18). The recombined BAC was injected into fertilized (CBA x B10)F1 oocytes. Analyses of T cells isolated from crosses to ROSA26-EYFP mice identified only 10% of Il9-producing cells were marked by EYFP. It was discovered that the IRES and mCherry in the construct was disabled because of a premature translational start site in the IRES sequence which possibly diminished activity from the ATG of mCherry sequence. (J:177640)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
