A loxP site was inserted upstream of exon 1. An FRT-flanked puro selection cassette with a 3' loxP site was inserted downstream of exon 1. Flp-mediated recombination removed the selection cassette. (J:176083)
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A loxP site was inserted upstream of exon 1. An FRT-flanked puro selection cassette with a 3' loxP site was inserted downstream of exon 1. Flp-mediated recombination removed the selection cassette. (J:176083)