Site-directed mutagenesis introduced scrambled mutations of the 20 bp CTCF binding site 1 (CBE1) and 19 bp CBE2 sites within the intergenic control region 1 (IGCR1). Cre-mediated recombination removed the neo cassette inserted into the IGCR1. (J:176649)
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Site-directed mutagenesis introduced scrambled mutations of the 20 bp CTCF binding site 1 (CBE1) and 19 bp CBE2 sites within the intergenic control region 1 (IGCR1). Cre-mediated recombination removed the neo cassette inserted into the IGCR1. (J:176649)