An inverted neo cassette was inserted upstream of exon 4 and a loxP site was inserted downstream of exon 5 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exons 4 and 5 floxed. (J:176655)
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An inverted neo cassette was inserted upstream of exon 4 and a loxP site was inserted downstream of exon 5 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exons 4 and 5 floxed. (J:176655)