RMCE with Gt(ROSA)26Sortm1(CAG-flpe)Arte inserted a construct containing: an F3 site, a neomycin cassette lacking a translation initiation site (ATG), a Pgk polyadenylation signal, the human promoter driving ubiquitous expression of a short hairpin RNA against Rad18 (shRNA 20-1), and an FRT site. (J:177475)