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An FRT flanked neomycin/kanamycin selection cassette and loxP site were inserted upstream of exon 4 and a loxP site was inserted downstream of exon 6 via homologous recombination. Cre mediated recombination deleted exons 4 - 6. RT-PCR and western blot analysis confirmed the absence of wild-type RNA and protein expression in testes and MEFs, respectively, from homozygous mice. RT-PCR analysis did detect expression of a small transcript containing exons 7 and 9 in testes from homozygous mice. (J:215857)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
