Tg(Thy1-APP*)2.2Ingm

The Thy1 regulatory sequence directs expression of a fusion protein of the pre-pro-peptide of murine thyrotropin releasing hormone and the N terminus of the modified human amyloid beta polypeptide, Abeta(Q3?42). This transgene expresses an N terminally truncated A beta protein that is glutamate to glutamine modified at position 3 and engineered to liberate its product preferentially within the secretory pathway. Multiple copies of the transgene inserted into Chr. 12 at 53.5 Mbp with a 2-4 kbp deletion. No effect on the expression of neighboring genes (Heatr5a, Arhgap5 and Akap6) was detected. Maximal transgene expression was seen between P12 and P28. (J:176575)