A loxP site was inserted upstream of exon 5 and an FRT-flanked puro cassette with a 3' loxP site was inserted downstream of exon 6. Flp-mediated recombination removed the puro cassette and left exons 5 and 6 floxed. (J:175827)
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A loxP site was inserted upstream of exon 5 and an FRT-flanked puro cassette with a 3' loxP site was inserted downstream of exon 6. Flp-mediated recombination removed the puro cassette and left exons 5 and 6 floxed. (J:175827)