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RedET recombineering was utilized to insert a cassette containing cre with a nuclear localization signal (NLS), a V5 epitope tag and the SV40 polyadenylation site, into a BAC clone (RP23-109K12) containing the advillin (Avil) locus. Correctly targeted clones were purified and injected into pronuclei of B6D2F1/N oocytes or prepared for intracytoplasmic sperm injection (ICSI). Both methods produced transgenic offspring. The line used for detailed analysis had 5 copies of the cre transgene. (J:176114)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
