An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the neo cassette leaving exon 3 floxed. (J:175422)
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An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the neo cassette leaving exon 3 floxed. (J:175422)