A targeting vector was designed to insert a loxP site upstream of exon 5, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 6. Flp-mediated recombination removed the neo cassette. (J:175748)
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A targeting vector was designed to insert a loxP site upstream of exon 5, and a frt-flanked neomycin resistance (neo) cassette followed by a second loxP site downstream of exon 6. Flp-mediated recombination removed the neo cassette. (J:175748)