A loxP site and an FRT flanked neo cassette were inserted 5' of exon 2 and a second loxP site were inserted 3' of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:174679)
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A loxP site and an FRT flanked neo cassette were inserted 5' of exon 2 and a second loxP site were inserted 3' of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette. (J:174679)