Tg(Eno2-Glrb)456Wha

The transgene contains the promoter of the rat enolase 2, gamma neuronal gene driving expression of a cDNA encoding the beta subunit of the rat glycine receptor, which is followed by the SV40 small T-antigen splice and polyadenylation signals. . Brains of both heterozygous and homozygous transgenic mice of line 456 on a homozygous Glrbspa background contain Glrb mRNA and membrane-bound glycine receptor alpha-1 subunit (a proxy for the beta receptor subunit), analyzed via mRNA northern and western blot analysis, respectively, at levels not significantly different from those in non-transgenic Glrbspa homozygotes. Despite this, binding of strychnine to spinal cord membrane preparations from the homozygous transgenic mice is consistently higher than to preps from the non-transgenic animals, though still quite low. (J:32212, J:60037)