A construct, which contained (5' to 3') an adenoviral splice acceptor site (SA); a wild-type FRT site, a floxed inverted luciferase cassette; a puromycin N-acetyltransferase (PAC) gene; a non-integrating FRT site; and a promoter and start-codon deficient neomycin phosphotransferase gene, was inserted into the locus. This allele is suitable for RMCE to replace the inverted luciferase gene with a gene of interest. (J:173806)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129P2/OlaHsd
Targeted
Insertion
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1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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