A loxP1 site was inserted upstream of exon 4. An FRT-flanked neo cassette with a 3' loxP2 site was inserted downstream of exon 4. Flpo-mediated recombination removed the neo cassette and left exon 4 floxed. (J:173909)
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A loxP1 site was inserted upstream of exon 4. An FRT-flanked neo cassette with a 3' loxP2 site was inserted downstream of exon 4. Flpo-mediated recombination removed the neo cassette and left exon 4 floxed. (J:173909)