Log In|Sign Up
EN
A targeting vector containing an FRT-site flanked NEO cassette and IRES-MerCreMer sequence was inserted in the 3' UTR of Oct4/Pou5f1 locus. The construct was electroporated into C57BL/6 derived embryonic stem (ES) cells from inGenious Targeting Laboratory. Correctly targeted ES cells were injected into recipient B6(Cg)-Tyrc-2J/J or BALB/c blastocysts. The resulting chimeric animals were crossed to B6.Cg-Tg(ACTFLPe)9205Dym/J mice to remove the FRT site flanked selection cassette. (J:194647)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
Show/Hide columns
| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
