An FRT-flanked cassette containing (5' to 3') a splice acceptor, IRES-lacZ, neo resistance gene, blasticidin resistance gene (BSD), and loxP site was inserted downstream of exon 3. A second loxP site was inserted upstream of exon 4. The absence of protein expression was confirmed by western blot analysis on testis extract. (J:173089)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count