An FRT flanked pgk-neo cassette and loxP sit were inserted into intron 4 and a loxP site was inserted into intron 7 via homologous recombination. Flp mediated recombination removed the pgk-neo cassette leaving exons 5-7 floxed. (J:172442)
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An FRT flanked pgk-neo cassette and loxP sit were inserted into intron 4 and a loxP site was inserted into intron 7 via homologous recombination. Flp mediated recombination removed the pgk-neo cassette leaving exons 5-7 floxed. (J:172442)