The firefly luciferase gene (from the pGL2 plasmid, Promega) was introduced into the ROSA26 locus using a knock-in strategy. The targeting vector was constructed to contain the coding sequence of firefly luciferase downstream of a loxP-flanked STOP cassette (consisting of a promoter-less neomycin gene followed by a three SV40 polyadenylation sequences).

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
129/Sv
Targeted
Insertion
--
--
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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