Exons 11 and 12 were replaced with a neo cassette via homologous recombination. Absence of mRNA and protein expression were confirmed by northern blotting, qRT-PCR, ELISA, and western blotting. (J:172218)
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Exons 11 and 12 were replaced with a neo cassette via homologous recombination. Absence of mRNA and protein expression were confirmed by northern blotting, qRT-PCR, ELISA, and western blotting. (J:172218)