An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 7. Flp-mediated recombination removed the neo cassette leaving exons 2 through 7 floxed. (J:170976)
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An FRT-flanked neo cassette with a 3' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 7. Flp-mediated recombination removed the neo cassette leaving exons 2 through 7 floxed. (J:170976)