An IRES-hAP and floxed PGK-neo cassette was inserted in exon 2 via homologous recombination. Western blot analysis confirmed the absence of protein expression in homozygous embryos. Alkaline phosphatase activity mirrors endogenous gene expression. (J:171767)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count