Sgce^{Gt(148G1)Cmhd}

The terminal exons (3' to exon 9) of Sgce were disrupted by the gene trap vector insertion. Homozygotes and heterozygotes with the paternally inherited allele show 60-70% reductions in expression of total Sgce. IAP-mass spectrometry and qRT-PCR shows that the major and brain-specific isoforms are eliminated while short isoforms terminating 5' to intron 9 and small amounts of a chimeric epsilon-sarcoglycan/beta-gal fusion protein are preserved. (J:251780)