Cspg4^tm1(cre)Akik

A targeting vector was designed so that it contained a homology arm-flanked nlsCre cDNA (nls;nuclear localization signal), a pgk-neo cassette 3' of the nlscre, and an MC1-driven diphtheria toxin selection gene. After homologous recombination in RENKA ES cells, targeted clones had the cre cDNA inserted immediately after the translational initiation codon of Cspg4. ES cell clones were injected into 8-cell CD1 embryos to produce germline chimeras to establish the knock-in line. (J:171287)