A loxP site was inserted into intron 2 and a floxed neo cassette was inserted into intro 3 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exon 3 floxed. (J:171346)
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A loxP site was inserted into intron 2 and a floxed neo cassette was inserted into intro 3 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exon 3 floxed. (J:171346)