A loxP site was inserted upstream of exon 1 and an FRT-loxP-neo-FRT-loxP cassette was introduced downstream of exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 1 floxed. (J:171226)
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A loxP site was inserted upstream of exon 1 and an FRT-loxP-neo-FRT-loxP cassette was introduced downstream of exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 1 floxed. (J:171226)