Tg(tetO-TPR/MET,-EGFP)12Tcre

A bi-directional tetracycline response element (TetO) drives expression of an enhanced green fluorescent protein reporter gene (EGFP) and of a cDNA encoding the human TRP-MET oncoprotein, in which the N-terminal of TPR (translocation promoter region), containing a pair of dimerization sites, is joined to the tyrosine kinase domain of the MET proto-oncogene, which is the receptor for hepatocyte growth factor. Crossing mice bearing this transgene with mice that express the tetracycline transactivator (tTA) or reverse transactivator (rtTA) protein under the control of a tissue-specific promoter permits regulation by tetracycline withdrawal/administration of the expression of TRP-MET and of the GFP reporter in the progeny. (J:120883)