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An enhancer trap lentiviral transgenic approach was used to generate these mice. A lentiviral vector transgene construct contained an EGFP/cre fusion gene under control of the mouse Thy1.2 minimal promoter (-270 to +30). The vector was designed with a self-inactivating lentiviral backbone (FUGW). The packaged lentivirus was injected subzonally (under the zona pellucida) into 1-2 cell stage C57BL/6J embryos. Founder mice from line 16255 were crossed to B6-Gt(ROSA)26Sortm1Sor animals to assess cre activity/transmission. Cre positive mice were subsequently bred to C57BL/6 mice to generate a cre positive, reporter negative line. (J:150856)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
