A loxP site was inserted upstream of exon 1, and a loxP site and an FRT-flanked neo cassette were introduced into intron 1 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 1 floxed. (J:170618)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count