Pcsk9^tm1.2Prat

The proximal promoter and exon 1 were flanked by loxP sites and an FRT flanked neomycin selection cassette was inserted just downstream of the loxP site in intron 1. The neomycin selection cassette was subsequently excised by flp recombinase expression after crossing to Gt(ROSA)26Sor mice and cre expression after crossing to Tg(CMV-cre)1Ipc mice resulted in excision of the proximal promoter and exon 1. (J:169834)