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EGFP was cloned into a 12.5 kb mouse Fadd minigene within exon 2 at the 3' end of the coding region. LoxP sites were also inserted upstream of exon 1 and downstream of exon 2. The final construct was injected into one-celled mouse embryos. Fifteen transgenic lines were produced. Western blot analysis detected the expected fusion protein in thymus, spleen, and lymph nodes. (J:113207)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
