A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette, leaving exons 2, 3, and 4 floxed. (J:169472)
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A loxP site was inserted upstream of exon 2. An FRT-flanked neo cassette with a 3' loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette, leaving exons 2, 3, and 4 floxed. (J:169472)