LoxP sites were inserted into intron 3 and 5. Flp-mediated recombination removed the FRT-flanked neo cassette, and cre-mediated recombination removed exons 4 and 5. The absence of transcript expression was confirmed by RT-PCR. (J:169481)
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LoxP sites were inserted into intron 3 and 5. Flp-mediated recombination removed the FRT-flanked neo cassette, and cre-mediated recombination removed exons 4 and 5. The absence of transcript expression was confirmed by RT-PCR. (J:169481)