Tg(Ncr1-icre)265Sxl

A cassette containing codon-improved cre recombinase (icre), an artificial intron, a bovine growth hormone polyadenylation signal, and an ampicillin-resistance gene flanked by FRT sites was recombined into the first exon of the Ncr1 gene contained in BAC RP23-267N11. Recombined BACs were electroporated with a plasmid expressing Flp to delete the ampicillin gene. The final construct was purified and injected into pronuclei of C57BL/6N oocytes. 4 founders (264-267) were generated with 3 demonstrating transgene expression. Line 265 was selected for detailed analysis. (J:169574)