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A cassette containing codon-improved cre recombinase (icre), an artificial intron, a bovine growth hormone polyadenylation signal, and an ampicillin-resistance gene flanked by FRT sites was recombined into the first exon of the Ncr1 gene contained in BAC RP23-267N11. Recombined BACs were electroporated with a plasmid expressing Flp to delete the ampicillin gene. The final construct was purified and injected into pronuclei of C57BL/6N oocytes. 4 founders (264-267) were generated with 3 demonstrating transgene expression. Line 265 was selected for detailed analysis. (J:169574)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
