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BAC clone RP23-308B16 was modified by recombineering methods so that a cre-polyA cassette with an FRT-ampicillin-FRT selection cassette replaced the start codon of Mlana (MART-1). Cre expression is controlled by the Mlana (MART-1) regulatory sequences. The targeting construct was electorporated into bacterial cells for homologous recombination and selection, followed by induction of Flp-mediated excision of the ampicillin cassette. The BAC construct was injected into fertilized oocytes and 5 founder lines, 4 of which showed reporter expression in pigment cells when crossed to ROSA26-R mice. Line 5 was propagated for futher analysis. (J:172135)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
