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An IRES-EGFP/cre with an FRT-flanked kanamycin selection cassette was targeted to exon 2 of the Wnt7a locus in BAC RP23-53E13. After homologous recombination in EL250 cells, correctly targeted clones were selected and FRT recombination was induced in cells by arabinose to remove the kanamycin cassette. The linearized BAC was injected into pronuclei of B6SJLF1 hybrid zygotes. Mice express an EGFP-cre fusion gene product. No line numbers are available. (J:167551, J:191769)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
