A loxP site was inserted upstream of the coding sequence, and an FRT-flanked neo cassette with a 3' loxP site was inserted downstream of the coding sequence. Cre-mediated recombination removed the coding sequence and the neo cassette. (J:166911)
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A loxP site was inserted upstream of the coding sequence, and an FRT-flanked neo cassette with a 3' loxP site was inserted downstream of the coding sequence. Cre-mediated recombination removed the coding sequence and the neo cassette. (J:166911)